A Synergistic Anti-Diabetic Effect by Ginsenosides Rb1 and Rg3 through Adipogenic and Insulin Signaling Pathways in 3T3-L1 Cells

Although ginsenosides Rb1 and Rg3 have been identified as the significant found in red ginseng that confer anti-diabetic actions, it is unclear whether insulin-sensitizing effects are mediated by individual compounds or their combination. To determine effect of on adipocyte differentiation, 3T3-L1 preadipocytes were induced to differentiate standard hormonal inducers absence presence Rg3. Additionally, we determined Rb1, Rg3, combination expression genes related adipogenic transcription factors, insulin signaling pathway cells using semi-quantitative RT-PCR. significantly increased CEBPα, PPARγ, aP2 mRNAs. However, exerted its maximal stimulatory these at 1 μM concentration, while a high concentration (50 μM) showed inhibitory effects. Similarly, treatment with (1 IRS-1, Akt, PI3K, GLUT4, adiponectin. Importantly, co-treatment (9:1) levels Our data indicate activity is, part, synergistic actions further supporting significance minor